Ramida Watanapokasin
Srinakharinwirot University, Thailand
Title: Anti-metastatic activity of Senna alata extract on SW1353 chondrosarcoma cancer cell line
Biography
Biography: Ramida Watanapokasin
Abstract
Statement of the Problem: Chondrosarcoma is the second most common malignant tumor of bone. The cancer originates from chondrocytes with abnormal proliferation and usually grows within a bone or on its surface. As 90% of human cancer death is due to metastasis process, in this study the anti-metastasis activity of Senna alata extract was studied in the highly metastasis SW1353 cell line. Senna alata is a medicinal plant which has been used in traditional folk medicine especially antimicrobial activity.
Methodology & Theoretical Orientation: S. alata extract was dissolved and diluted in DMSO at the desired concentrations. MTT assay was used to investigate the effects of S. alata extract on cell proliferation and cell growth. To study the effects of S. alata extract on cell migration and invasion, wound healing assay and transwell migration assay were performed, respectively. Signaling proteins were observed using Western blot analysis.
Findings: Our study found that at sub-toxic dose of S. alata extract inhibited cell migration and cell invasion in SW1353 treated cells. Moreover, Western blot analysis indicated that S. alata extract down-regulated p-ERK1/2 and p-Akt (S473) that involved in cell growth and proliferation.
Conclusion & Significance: S. alata extract showed the effect on metastasis inhibition in SW1353 treated-cells. In addition, S. alata extract could inhibit cell growth and proliferation via mediator proteins, ERK1/2 and Akt. These finding could be useful in the development of S. alata extract to be a metastasis inhibition agent.